Research is fundamental to our success. The research collaboration between BioClin and the Free University medical center of Amsterdam revealed the anti-adhesive mode of action of the large, negatively-charged polysaccharides derived from the leaf gel of Aloe Barbadensis Miller. This resulted in a shared, global patent for 2QR-complex and the development of BioClin’s first product, Multi-Gyn ActiGel. The following studies demonstrate the anti-adhesive properties of 2QR-complex and show how effective this mode of action is at blocking the progress of pathogenic microbes.
Our research program for 2QR-complex continues today at the University of Applied Sciences in Utrecht, The Netherlands. We are currently developing randomized, placebo-controlled clinical trials to demonstrate the efficacy of our products and add to our evidence base. We also work with our international partners to assess the efficacy of our products with healthcare professionals and patients around the world.
Advantages of 2QR-complex: scientific evidence
Anti-adhesion activity of 2QR-complex on various natural substrates
Glass microscope slides showing the microbial adhesion of C. albicans and S. aureus on gelatin, poly-L-lysine and epithelial cells were tested with 2QR-complex. Gelatin, poly-L-lysine and epithelial cells are biological protein substrates which microbes can adhere to. Control slides with the same natural substrates and bacteria were incubated without 2QR-complex.
The slides demonstrate the anti-adhesive action of 2QR complex:
80-90% reduction in microbial adhesion to poly-L-lysine and gelatin.
60% reduction in microbial binding to epithelial cells.
2QR-complex does not affect the viability of microbes
Various microbial species were incubated in growth medium in the absence and presence of 2QR-complex. After 1 hour of incubation, the number of surviving cells was determined quantitatively (total plate count). Subsequently, the incubations were allowed to grow overnight, and the amount of microbial growth was determined in a turbidity measurement, ‘optical density’ (OD).
For all microorganisms tested, the numbers of colony-forming units after 1 hour incubation with purified 2QR-complex was similar to control incubations without 2QR-complex. Moreover, after overnight incubation the extent of microbial growth in the presence and absence of 2QR-complex polysaccharides were highly comparable. In conclusion, these results convincingly show 2QR-complex polysaccharides do not affect microbial viability and do not affect microbial growth in vitro.